Vanilloid R-like 3/TRPV3 antibody
Boster Bio Anti-TRPV3 Antibody Picoband™ catalog # A03874-1. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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Product Info Summary
| SKU: | A03874-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-TRPV3 Antibody Picoband™
View all Vanilloid R-like 3/TRPV3 Antibodies
SKU/Catalog Number
A03874-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPV3 Antibody Picoband™ catalog # A03874-1. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPV3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03874-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV3 recombinant protein (Position: E28-V790).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03874-1 is reactive to TRPV3 in Human, Mouse, Rat
Applications
A03874-1 is guaranteed for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
127459 MW
Background of Vanilloid R-like 3/TRPV3
TRPV3 (Transient Receptor Potential Cation Channel Subfamily V Member 3), also known as VRL3, is a human gene encoding the protein of the same name. The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. Peier et al. localized the TRPV3 gene to a BAC clone mapped to chromosome 17p13. They mapped the mouse gene to chromosome 11B4. Peier et al. stably expressed mouse Trpv3 in Chinese hamster ovary cells and assayed electrophysiologic activity by whole cell voltage-clamp techniques. They determined that Trpv3 is a cation-permeable channel activated by warm and hot temperatures. Xu et al. showed that increasing temperature from approximately 22 to 40 degrees Celsius in mammalian cells transfected with human TRPV3 elevated intracellular calcium by activating a nonselective cationic conductance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. IHC analysis of TRPV3 using anti-TRPV3 antibody (A03874-1).
TRPV3 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV3 Antibody (A03874-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of TRPV3 using anti-TRPV3 antibody (A03874-1).
TRPV3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV3 Antibody (A03874-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow Cytometry analysis of A431 cells using anti-TRPV3 antibody (A03874-1).
Overlay histogram showing A431 cells stained with A03874-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV3 Antibody (A03874-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 4. Flow Cytometry analysis of CACO-2 cells using anti-TRPV3 antibody (A03874-1).
Overlay histogram showing CACO-2 cells stained with A03874-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV3 Antibody (A03874-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 5. Western blot analysis of TRPV3 using anti-TRPV3 antibody (A03874-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MDA-MB-453 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV3 antigen affinity purified polyclonal antibody (Catalog # A03874-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV3 at approximately 100KD. The expected band size for TRPV3 is at 91KD.
Gene/Protein Information For TRPV3 (Source: Uniprot.org, NCBI)
Gene Name
TRPV3
Full Name
Transient receptor potential cation channel subfamily V member 3
Weight
127459 MW
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
transient receptor potential cation channel subfamily V member 3; transient receptor potential cation channel, subfamily V, member 3; TRPV3; vanilloid receptor 3; Vanilloid receptor-like 3; vanilloid receptor-related osmotically activated channel protein; Vanilloid R-like 3; VRL3; VRL-3 TRPV3 FNEPPK2, OLMS, OLMS1, VRL3 transient receptor potential cation channel subfamily V member 3 transient receptor potential cation channel subfamily V member 3|VRL-3|vanilloid receptor-like 3|vanilloid receptor-related osmotically activated channel protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on TRPV3, check out the TRPV3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPV3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [emailprotected].
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Compatible Secondaries For Host Rabbit
- HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
- Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugated (BA1003)
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Positive Controls
Blocking peptides and recombinant immunogen proteins are available at $150 per 1mg. Contact [emailprotected] for a quote.
Western blot reagents
- SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112)
- Western Blotting Filter Paper, 0.158mm thick, 9cm × 7.5cm (AR0173)
- Cytoplasmic and Nuclear Protein Extraction Kit (AR0106)
- Broad Spectrum Protease Inhibitor co*cktail (100x) (AR1182)
- See all Western blot reagents
Immunohistorychemistry/IHC reagents
- 4% Paraformaldehyde (PFA) Solution in PBS (with DEPC) (AR1068)
- Poly-L-Lysine Coated Slides (AR1065)
- DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining (AR1176)
- See all IHC reagents
- See featured antibodies and ELISA kits
Specific Publications For Anti-TRPV3 Antibody Picoband™ (A03874-1)
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